By using a pattern matching analysis integrated into PicoQuant's SymPhoTime 64 software, excellent FLIM contrast can be obtained without need for classic decay data fitting. The rapidFLIM method can be integrated into many existing confocal laser scanning microscopes from major manufacturers such as Nikon, Olympus, or Zeiss through LSM upgrade kits offered by PicoQuant.

PicoQuant's wide range of products for photon counting includes several high-end modules for time-correlated single photon counting (TCSPC) and event timing, single photon sensitive detectors and specialized analysis software for the evaluation of (time-resolved) fluorescence measurements and quantum correlations.

It is not meant to be complete. All TCSPC modules provided by PicoQuant expect standard TTL pulses for the external markers. This means 2-5 V for a logic “high”-level and 0-0.8 V for a logic “low”-level. For more info about TTL … The compilation below is probably not complete. If you are the maintainer of a software that deals with TCSPC data please edit this page and add your software listing. Alternatively you can also use https://support.picoquant.com to contact PicoQuant support to submit your software project. libpicoquant.

Result files also contain a comment which can be displayed via the file menu: File → Show Comment. Typically the result-file opens with a preview display (Image, FCS-traces, TCSPC-curve or Time Trace - … Time-correlated single photon counting (TCSPC) is a powerful method to accurately measure the fluorescence lifetime of samples. We use a TCSPC setup from Picoquant. Using a laser pulsed at 40 MHz ( PDL-800-B ), the light intensity is reduced to the level where the photomultiplier detects a photon only after 1% of laser pulses ( i.e., count rate <0. PicoQuant releases first TCSPC system with USB 3.0 09 Aug 2012 The new “HydraHarp 400” is now qualified for most advanced high throughput applications and facilitates life science work as well as quantum information processing. PicoHarp 300 PicoQuant Time-Correlated Single Photon Counting (TCSPC) system PicoQuant The PicoHarp 300 is a high-end, easy to use, plug and play Time-Correlated Single Photon Counting (TCSPC) system.

PicoQuant's new TCSPC and MCS board TimeHarp 260 is a compact, easy to use system for the PCI Express interface 06 Mar 2013 PicoQuant has released a new Time-Correlated Single Photon Counting (TCSPC) and Multichannel Scaling (MCS) board with PCI Express (PCIe) interface.

ZERO BIAS - scores, article reviews, protocol conditions and more PicoQuant's wide range of products for photon counting includes several high-end modules for time-correlated single photon counting (TCSPC) and event timing, single photon sensitive detectors and specialized analysis software for the evaluation of (time-resolved) fluorescence measurements. prepare 1mL of saturated water solution of KI (potassium iodide) add 0.17 mL of saturated water solution of Erythrosine B (at least 95% of purity) add 0.03 mL of 0.004 M KOH (potassium hydroxide) solution in order to achieve pH10. Storage: keep at ~ 4°C, renew the solution after one month.

All TCSPC modules provided by PicoQuant expect standard TTL pulses for the external markers. This means 2-5 V for a logic “high”-level and 0-0.8 V for a logic “low”-level. For more info about TTL signals see here.. Please go to manual of the TCSPC module

prepare 1mL of saturated water solution of KI (potassium iodide) add 0.17 mL of saturated water solution of Erythrosine B (at least 95% of purity) add 0.03 mL of 0.004 M KOH (potassium hydroxide) solution in order to achieve pH10. Storage: keep at ~ 4°C, renew the solution after one month. Spectra: PicoQuant's wide range of products for photon counting includes several high-end modules for time-correlated single photon counting (TCSPC) and event timing, single photon sensitive detectors and specialized analysis software for the evaluation of (time-resolved) fluorescence measurements. It contains the complete optics and electronics for recording fluorescence decays by means of Time-Correlated Single Photon Counting (TCSPC) and/or Multi-Channel Scaling (MCS). The system is optimized for high temporal resolution and can be used with femtosecond or picosecond lasers, like the picosecond diode lasers from PicoQuant.

Current file format (.ptu) and is subsequent to the former .pt2 or .pt3 and can handle both T2 and T3 acquisition modes for a variety of TCSPC devices (MultiHarp, HydraHarp, TimeHarp, PicoHarp, etc.) The PicoHarp 300 is a high-end, easy to use, plug and play Time-Correlated Single Photon Counting (TCSPC) system. A special design approach provides identical and synchronized but independent input channels. They can be used as detector inputs for coincidence correlation experiments or as a pair of start and stop inputs for TCSPC.
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It is also worth noting that by virtue of quantum mechanics the actual count arrival times are random, The HydraHarp 400 is a high-end, easy to use, plug and play Time Interval Analyzer (TIA) and Time-Correlated Single Photon Counting (TCSPC) system with scalability for multiple channels (covered by patent DE 10 2008 004 549). It is connected to a PC through a USB 3.0 high speed interface. This wiki is a loose collection of documents that the Support team of PicoQuant found to be of general interest to our customers or people working with Time Resolved Fluorescence or TCSPC in general. It is not meant to be complete.

BERLIN, Feb. 24 -- A new driver library for custom programming is now available to run the compact and easy-to-use TimeHarp TCSPC board from PicoQuant Time-correlated single photon counting (TCSPC) is a powerful method to accurately We use the TimeHarp software by Picoquant for TCSPC experiments. 28 Jan 2021 2021), PicoQuant will unveil its latest multichannel event timer: The MultiHarp 160 – a scalable multichannel event timer and TCSPC unit. SPC-530 TCSPC; MSA-100 1ns multiscaler; MSA-300 5ns Picosecond laser pulses from a 670 nm and a 785 nm diode laser (PicoQuant, Berlin) were Поставщиками компании АЗИМУТ ФОТОНИКС являются такие ведущие мировые производители оптоэлектронных компонентов как Thorlabs, AiT Unter zeitkorrelierter Einzelphotonenzählung (englisch time-correlated single photon counting, TCSPC) versteht man eine (PicoQuant GmbH, PDF-Datei); Technical Note TCSPC.
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Today recording of T3-mode and T2-mode files is possible with all recent PicoQuant TCSPC devices (TimeHarp 260, PicoHarp 300 and HydraHarp 400 and their included software, analysis is supported mainly by the SymPhoTime software package.

A confocal-based multi-acquisition system, TCS SP8 from Leica with a TCSPC (HydraHarp 400, PicoQuant, Berlin, Germany), was used to measure the fluorescence lifetimes. PicoQuant - Fluorescence Lifetime Systems, Picosecond Diode Lasers, TCSPC Systems Ultraviolet to Infrared Picosecond Diode Laser Heads Voted Top Ten at CLEO 2000 LDH 375 to 1550 Laser Head Specifications (typical values) Share your videos with friends, family, and the world